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batf anf jun  (OriGene)


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    Structured Review

    OriGene batf anf jun
    Batf Anf Jun, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/batf+anf+jun/c-Jun+(JUN)+(NM_002228)+Human+Tagged+ORF+Clone/10__7554_slash_elife__32340-382-0-6
    Average 93 stars, based on 15 article reviews
    batf anf jun - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Western Blot:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Control:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Luciferase:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Activity Assay:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Electrophoretic Mobility Shift Assay:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Incubation:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Labeling:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Isolation:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Purification:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Construct:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Generated:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Recombinant:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Sequencing:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    TA Cloning:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Expressing:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Mutagenesis:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Staining:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Migration:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.

    Software:

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: cDNA of IRF4 and PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific). .. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively). .. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Tech

    Article Title: IRF4 haploinsufficiency in a family with Whipple’s disease
    Article Snippet: The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).The full-length cDNA of IRF4 and  PU.1 was inserted into the pcDNA 3.1D/V5-His-TOPO vector with the directional TOPO expression kit (Thermo Fisher Scientific).. BATF anf JUN were obtained from Origene companie (#RC207104 and #RC209804, respectively).. Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.Constructs carrying mutant alleles were generated from this plasmid by mutagenesis with a site-directed mutagenesis kit (QuikChangeII XL; Agilent Technologies), according to the manufacturer’s instructions.



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